期刊
ISCIENCE
卷 11, 期 -, 页码 246-+出版社
CELL PRESS
DOI: 10.1016/j.isci.2018.12.017
关键词
-
资金
- Belgian Fonds de la Recherche Scientifique - FNRS
- Belgian Science Policy (ILJAP-VII network) [P7/07]
- Fondation Leon Fredericq (FLF, Faculty of Medicine-University of Liege)
- FLF
- Fonds pour la formation a la Recherche dans l'Industrie et l'Agriculture (FRIA)
- Fonds de la Recherche Scientifique - FNRS
The cochlear sensory epithelium contains a functionally important triangular fluid-filled space between adjacent pillar cells referred to as the tunnel of Corti. However, the molecular mechanisms leading to local cell-cell separation during development remain elusive. Here we show that EphA4 associates with ADAM10 to promote the destruction of E-cadherin-based adhesions between adjacent pillar cells. These cells fail to separate from each other, and E-cadherin abnormally persists at the pillar cell junction in EphA4 forward-signaling-deficient mice, as well as in the presence of ADAM10 inhibitor. Using immunolabeling and an in situ proximity ligation assay, we found that EphA4 forms a complex with E-cadherin and its sheddase ADAM10, which could be activated by ephrin-B2 across the pillar cell junction to trigger the cleavage of E-cadherin. Altogether, our findings provide a new molecular insight into the regulation of adherens junctions, which might be extended to a variety of physiological or pathological processes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据