期刊
FOOD & FUNCTION
卷 10, 期 1, 页码 304-314出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8fo01991f
关键词
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资金
- National 13th Five-Year Plan for Science Technology [2016YFNC010104, 2016YFD0400505]
- Innovative Research Team of Tianjin Municipal Education Commission [TD13-5013]
- National 12th Five-Year Plan for Science Technology [2012BAD33B04]
- State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science Technology [17YDLJNC00130, 17PTSYJC00080, 18ZYPTJC00020]
- Project program of Key Laboratory of Food Nutrition and Safety, Ministry of Education, China [2018013]
- State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin, P.R. China [SKLFNS-KF-201811]
A water-soluble polysaccharide (BP-1) was extracted and purified from highland barley (Hordeum vulgare L.) and its average molecular weight was about 6.7 x 10(4) Da. In this study, the immunomodulatory activity of BP-1 on the immunosuppressive BALB/c mice model and its molecular mechanism were elucidated. It was found that the weight indexes of spleen and thymus were significantly increased by BP-1 (80 mg kg(-1) and 160 mg kg(-1)) treatment in the immunosuppressive mice model. The results showed that BP-1 (80 mg kg(-1) and 160 mg kg(-1)) could significantly increase the number of bone marrow cells (BMC) and peripheral blood white blood cells (WBC) in the immunosuppressive mice model. In addition, the result further confirmed that BP-1 could increase the serum levels of IL-2, TNF-alpha and IFN-gamma, so as to improve the immune function of immunosuppressive mice. The results showed that BP-1 (80 mg kg(-1) and 160 mg kg(-1)) could promote the proliferation of spleen cells and the natural killer (NK) cell activity in vivo. The quantitative real-time polymerase chain reaction (qRT-PCR) and ELISA results revealed that BP-1 (80 mg kg(-1) and 160 mg kg(-1)) could enhance the production of IL-2, TNF-a, IFN-gamma, IgG and IgM in the spleen of immunosuppressive mice. The HE (hematoxylin and eosin) stained histopathological images showed that BP-1 (80 mg kg(-1) and 160 mg kg(-1)) could repair the damage induced by CTX in the spleen cells of immunosuppressive mice. The result of macrophages showed that BP-1 (80 mg kg(-1) and 160 mg kg-1) could promote the proliferation and phagocytosis activity of macrophages in immunosuppressive mice. Furthermore, BP-1 could activate macrophages by the TLR-4, TRAF6, TAK1 and nuclear factor kappa B (NF-kappa B) p65 pathways in vivo. These results suggested that BP-1 has a remarkable immunomodulatory activity on the immunosuppressive mice model.
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