期刊
NATURE PLANTS
卷 5, 期 1, 页码 14-17出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/s41477-018-0321-8
关键词
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资金
- Cabinet Office, Government of Japan
- Cross-ministerial Strategic Innovation Promotion Program (SIP), Technologies for creating next-generation agriculture, forestry and fisheries (funding agency: Biooriented Technology Research Advancement Institution, NARO)
Streptococcus pyogenes Cas9 (SpCas9) is widely used for genome editing and requires NGG as a protospacer adjacent motif (PAM). Here, we show that the engineered SpCas9 (SpCas9-NGv1) can efficiently mutagenize endogenous target sites with NG PAMs in the rice and Arabidopsis genomes. Furthermore, we demonstrate that the SpCas9-NGv1 nickase fused to cytidine deaminase mediates C-to-T substitutions near the 5' end of the target sequence.
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