期刊
BIOMOLECULES
卷 9, 期 1, 页码 -出版社
MDPI
DOI: 10.3390/biom9010004
关键词
Fmoc-Pexiganan; NO release; antimicrobial activity; collagen
资金
- FEDER (Fundo Europeu de Desenvolvimento Regional) funds via COMPETE 2020 (Operacional Programme for Competitiveness and Internationalisation (POCI), Portugal 2020)
- Portuguese funds through FCT (Fundacao para a Ciencia e a Tecnologia/Ministerio da Ciencia, Tecnologia e Ensino Superior) [POCI-01-0145-FEDER-007274, PTDC/QUI-QFI/29914/2017, SFRH/BD/84914/2012]
- FCT [UID/QUI/5006/2013]
- Fundação para a Ciência e a Tecnologia [PTDC/QUI-QFI/29914/2017, SFRH/BD/84914/2012] Funding Source: FCT
Nitric oxide (NO) is an endogenously produced molecule that has been implicated in several wound healing mechanisms. Its topical delivery may improve healing in acute or chronic wounds. In this study an antimicrobial peptide was synthesized which self-assembled upon a pH shift, forming a hydrogel. The peptide was chemically functionalized to incorporate a NO-donor moiety on lysine residues. The extent of the reaction was measured by ninhydrin assay and the NO release rate was quantified via the Griess reaction method. The resulting compound was evaluated for its antimicrobial activity against Escherichia coli, and its effect on collagen production by fibroblasts was assessed. Time-kill curves point to an initial increase in bactericidal activity of the functionalized peptide, and collagen production by human dermal fibroblasts when incubated with the NO-functionalized peptide showed a dose-dependent increase in the presence of the NO donor within a range of 0-20 mu M.
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