Melamine-modified Boron-doped Diamond towards Enhanced Detection of Adenine, Guanine and Caffeine

This work describes the electrochemical method of boron-doped diamond (BDD) modification by poly-melamine to obtain organic films. The detection of adenine, guanine and caffeine was carried out by differential pulse wave voltammetry (DPV). The poly-melamine modified B-NCD electrodes exhibit excellent activity towards the electrochemical oxidation of all examined analytes. The poly-melamine modified BDD electrodes in all measurements exhibit a larger peak current and are more sensitive compared to the unmodified BDD electrodes. The detection limit for adenine, guanine and caffeine using poly-melamine modified BDD electrodes was found with 0.2 mu M, 0.02nM and 0.4 mu M, respectively. Moreover, the double-stranded (ctDNA), and single stranded DNA from calf thymus (ssDNA) was detected by DPV.