期刊
MICROCHIMICA ACTA
卷 184, 期 5, 页码 1445-1451出版社
SPRINGER WIEN
DOI: 10.1007/s00604-017-2138-y
关键词
Aptasensor; Antibiotics; Electrocatalysis; Linear sweep voltammetry; Milk analysis
资金
- National Natural Science Foundation of China [21475033]
- Natural Science Foundation of Hubei Province of China [2014CFB279]
- Scientific Research Foundation for the Returned Overseas Chinese Scholars, Ministry of Education of China [2015-49]
- Science Foundation for Creative Research Group of HBNU [T201501]
A nanocomposite prepared from reduced graphene oxide (rGO) and silver nanoparticles (AgNPs) is used in an electrochemical aptasensor for the sensitive and selective determination of the antibiotic chloramphenicol (CAP). The nanocomposite was obtained by electrostatic assembly of AgNPs on the surface of polyelectrolyte-functionalized rGO and then used to modify a glassy carbon electrode. The biosensor is then obtained by immobilizing the aptamer against CAP. When incubated with solutions of CAP, the sensor surface is loaded with CAP due to aptamer recognition. The captured CAP can be electrochemically reduced to yield a current that is strongly enhanced as a result of the excellent electrocatalysis property of the graphene/AgNP-nanocomposite. Under optimum conditions, the calibration plot is linear in the 0.01 to 35 mu M concentration range, with a 2 nM detection limit (at 3 sigma). The sensor is reproducible, stable, selective over homologous interferents, and performs excellently when analyzing CAP in milk samples.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据