Preparation of porous methacrylate monoliths with oxidized single-walled carbon nanohorns for the extraction of nonsteroidal anti-inflammatory drugs from urine samples

A copolymer was prepared from glycidyl methacrylate, ethylene glycol dimethacrylate and oxidized single-walled carbon nanohorns via photo-polymerization and used in spin columns for the extraction of nonsteroidal anti-inflammatory drugs (NSAIDs) from human urine samples. All microextraction procedures (loading, washing and elution) can be performed by centrifugation. The hybrid monolithic polymers were characterized by scanning electron microscopy and nitrogen intrusion porosimetry. Following elution with methanol, the NSAIDs (naproxen, fenbufen, flurbiprofen, and ibuprofen) were quantified by reversed-phase HPLC with UV detection. The detection limits varied between 0.1 and 10 mu g center dot L-1, and the precision (relative standard deviation) ranged from 3.5 to 11.8%. Relative recoveries between 81 and 106% were found when analyzing spiked urine samples.