期刊
MICROBIOLOGICAL RESEARCH
卷 203, 期 -, 页码 57-67出版社
ELSEVIER GMBH
DOI: 10.1016/j.micres.2017.06.008
关键词
Fungicide resistance; Host pathogen interaction; Penicillium digitatum; Sucrose transporter; Virulence
类别
资金
- Ministry of Science and Innovation (Spain) [AGL2008-04828-C03, ALG2001-30519-C03-02]
- Institut Nacional de Lnvestigaciones Agrarias (INIA)
A putative sucrose transporter PdSUT1 included in the same clade that Sutlp from Schizosaccharomyces pombe was identified in Penicillium digitatum, the major citrus postharvest pathogen. PdSUT1 gene was characterized using target gene disruption and gene overexpression. The Delta PdSUT1 mutants generated by gene elimination showed reduction in fungal virulence during citrus fruit infection assayed in mature fruit at 20 degrees C. However, the overexpression mutants did not increased disease severity neither in the mutants coming from a high virulent nor from a low virulent P. digitatum progenitor strains. Moreover, fungicide sensitivity was affected in the deletant mutants but not in the overexpression transformants. The expression analysis of several genes involved in fungicide resistance showed an intensification of MFS transporters and a decrease of sterol demethylases transcriptional abundance in the Delta PdSUT1 mutants compare to the parental wild type strain. PdSUT1 appear not to be directly involved in fungicide resistance although can affect the gene expression of fungicide related genes. These results indicate that PdSUT1 contribute to P. digitatum fungal virulence and influence fungicide sensitivity through carbohydrate uptake and MFS transporters gene activation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据