期刊
MICROBIAL PATHOGENESIS
卷 112, 期 -, 页码 63-69出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2017.09.047
关键词
Termite gut; PY12; MX5; LiP; 16S rRNA; Recombinant Lactococcus lactis
资金
- National Key Research and Development Program of China [2016YFD0501409-03]
- National Natural Science Foundation of China [NSFC:31372331]
- China Agriculture Research System [CARS-38]
- Special Fund for Agro-scientific Research in the Public Interest [201303091]
Lignin, a common natural polymers, is abundant and complex, and termites can break down and utilize the lignin in their food. In this study an attempt was made to isolate and characterize the lignolytic bacteria from termite (Reticulitermes chinensis Snyder) gut. Two strains (PY12 and MX5) with high lignin peroxidase (LiP) activity were screened using the azure B method. By analyzing their 16S rRNA, the strain PY12 was classified as Enterobacter hormaechei; MX5, as Bacillus licheniformis. We then optimized the different conditions of liquid fermentation medium, and obtained LiP activities of 278 U/L and 256 LI/L for PY12 and MX5, respectively. Subsequently, we confirmed the LiP activities of the strains by evaluating their decolorizing effects on various dyes. Finally, we cloned the LiP gene of strain PY12 and successfully transferred it to Lactococcus lactis. We believe that our results provide the theoretical and practical basis for the production of genetically engineered bacteria that produce LiP, thus allowing for the utilization of naturally available lignin as an energy resource. (c) 2017 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据