期刊
MICROBIAL PATHOGENESIS
卷 104, 期 -, 页码 125-132出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.micpath.2017.01.023
关键词
TTO; Nanotechnology; Biofilm; Candida; Calcofluor white
资金
- PPGPE/Centro Universitario Franciscano-Probic
- CNPq
- CAPES
- FAPERGS
Candida infection is an important cause of morbidity and mortality on immunosuppressed patients. This growing trend has been associated with resistance to the antimicrobial therapy and the ability of microorganism to form biofilms. TTO oil is used as antimicrobial which shows antibiofilm activity against Candida species. However, it presents problems due to its poor solubility and high volatility. The present study aimed to evaluate in vitro antibiofilm activity of TTO nanoparticles against many Candida species. It was performed the characterization of the oil and nanoparticles. The levels of exopolysaccharides, proteins, and the biomass of biofilms were measured. The chromatographic profile demonstrated that the TTO oil is in accordance with ISO 4730 with major constituents of 41.9% Terpinen-4-ol, 20.1% of gamma-Terpinene, 9,8% of alpha-Terpinene, and 6,0% of 1,8-Cineole. The TTO nanoparticles showed pH of 63, mean diameter of 158.2 +/- 2 nm, polydispersion index of 0.213 +/- 0.017, and zeta potential of -8.69 +/- 0.80 mV. The addition of TTO and its nanoparticles represented a significant reduction of biofilm formed by all Candida species, as well as a reduction of proteins and exopolysaccharides levels. It was possible to visualize the reduction of biofilm in presence of TTO nanoparticles by Calcofluor White method. (C) 2017 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据