期刊
MICROBIAL DRUG RESISTANCE
卷 23, 期 7, 页码 822-825出版社
MARY ANN LIEBERT, INC
DOI: 10.1089/mdr.2016.0245
关键词
qnr; cytotoxic effect; E.coli
资金
- Ministerio de Sanidad y Consumo, Instituto de Salud Carlos III [PI11-00934, PI14/00940]
- Consejeria de Innovacion Ciencia y Empresa, Junta de Andalucia, Spain [P11-CTS-7730]
- Plan Nacional de I+D+i
- Instituto de Salud Carlos III, Subdireccion General de Redes y Centros de Investigacion Cooperativa, Ministerio de Economia y Competitividad
- Spanish Network for Research in Infectious Diseases [REIPI RD12/0015]
- European Regional Development Fund A way to achieve Europe'' ERDF
Objective: The objective was to evaluate the cytotoxic effect associated with overexpression of multiple Qnr-like plasmid-mediated quinolone resistance (PMQR) mechanisms in Escherichia coli. Methods: Coding regions of different PMQR genes (qnrA1, qnrB1, qnrC, qnrD1, qnrS1, and qepA2) and efsqnr were cloned into pET29a(+) vector and overexpressed in E. coli BL21. E. coli BL21 with and without an empty pET29a(+) vector were used as controls. The cytotoxic effect associated with PMQR mechanism overexpression was determined by transmission electron microscopy and viability assays. Results: Overexpressed qnr genes produced loss of bacterial viability in the range of 77-97% compared with the controls, comparable with loss of viability associated with EfsQnr overexpression (97%). No loss of viability was observed in E. coli overexpressing QepA2. In transmission electron microscopy assays, signs of cytotoxicity were observed in E. coli cells overexpressing EfsQnr and Qnr proteins (30-45% of the bacterial population showed morphological changes). Morphological changes were observed in less than 5% of bacterial populations from the control strains and E. coli overexpressing QepA2. Conclusions: Overexpression of qnr genes produces a cytotoxic cellular and structural effect in E. coli, the magnitude of which varies depending on the family of Qnr proteins.
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