期刊
MICROBIAL CELL FACTORIES
卷 16, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s12934-017-0723-8
关键词
Delta-6 desaturase; Delta-12 desaturase; GLA production; Homologous overexpression; Mucor circinelloides
资金
- National Natural Science Foundation of China [31670064, 31271812]
- Science and Technology Project of Shandong College [J16LE20]
- China Postdoctoral Science Foundation [2017M612305, 2015T80735]
- Doctor and Postdoctoral Foundation of Shandong University of Technology [4041/415047, 4041/516002]
- Taishan Industry Leading Talent Project
Background:Y-Linolenic acid (GLA) is important because of its nutritional value and medicinal applications. Although the biosynthetic pathways of some plant and microbial GLA have been deciphered, current understanding of the correlation between desaturases and GLA synthesis in oleaginous fungi is incomplete. In previous work, we found that a large amount of oleic acid (OA) had not been converted to linoleic acid (LA) or GLA in Mucor circinelloides CBS 277.49, which may be due to inadequate activities of the delta-12 or delta-6 desaturases, and thus leading to the accumulation of OA and LA. Thus, it is necessary to explore the main contributing factor during the process of GLA biosynthesis in M. circinelloides. Results: To enhance GLA production in M. circinelloides, homologous overexpression of delta-12 and two delta-6 desaturases (named delta-6-1 and delta-6-2, respectively) were analyzed. When delta-6 desaturase were over-expressed in M. circinelloides, up to 43% GLA was produced in the total fatty acids, and the yield of GLA reached 180 mg/l, which were, respectively, 38 and 33% higher than the control strain. Conclusion: These findings revealed that delta-6 desaturase (especially for delta-6-1 desaturase) plays an important role in GLA synthesis by M. circinelloides. The strain overexpressing delta-6-1 desaturase may have potential application in microbial GLA production.
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