期刊
METHODS
卷 116, 期 -, 页码 95-111出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2016.11.010
关键词
Site-selective; Site-specific; Orientation; Immobilization; Antibody; Antibody fragments; Click chemistry; Affinity tags; Protein A; Nucleotide binding protein; Metal binding protein; Staudinger ligation; Cycloaddition; Antibody-binding protein; Physical adsorption; Diagnostics; Therapeutics; Separation
资金
- National Institute of Biomedical Imaging and Bioengineering (NIBIB) [EB016707, EB014586]
- NIH
Immobilized antibody systems are the key to develop efficient diagnostics and separations tools. In the last decade, developments in the field of biomolecular engineering and crosslinker chemistry have greatly influenced the development of this field. With all these new approaches at our disposal, several new immobilization methods have been created to address the main challenges associated with immobilized antibodies. Few of these challenges that we have discussed in this review are mainly associated to the site-specific immobilization, appropriate orientation, and activity retention. We have discussed the effect of antibody immobilization approaches on the parameters on the performance of an immunoassay. (C) 2016 Elsevier Inc. All rights reserved.
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