期刊
METHODS
卷 126, 期 -, 页码 76-85出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2017.05.027
关键词
Microinjection; Fluorescent in situ hybridization; mRNA nuclear export kinetics; Mammalian tissue culture cells; Gene expression
资金
- Canadian Institutes of Health Research [FRN 102725]
- Natural Sciences and Engineering Research Council of Canada [RGPIN-2016-06607]
- Ontario Graduate Scholarship
The nuclear export of mRNAs is an important yet little understood part of eukaryotic gene expression. One of the easiest methods for monitoring mRNA export in mammalian tissue culture cells is through the microinjection of DNA plasmids into the nucleus and monitoring the distribution of the transcribed product over time. Here we describe how to setup a microscope equipped with a micromanipulator used in cell microinjections, and we explain how to perform a nuclear mRNA export assay and obtain the nuclear export rate for any given mRNA. (C) 2017 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据