期刊
METHODS
卷 117, 期 -, 页码 28-34出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ymeth.2016.11.011
关键词
MS2-affinity purification; RNA sequencing (RNAseq); Small RNAs (sRNAs); Targetome; tRNA derived fragments (tRFs)
资金
- Canadian Institutes of Health Research (CIHR)
Recent advances in high-throughput sequencing have led to an explosion in the rate of small regulatory RNAs (sRNAs) discovery among bacteria. However, only a handful of them are functionally characterized. Most of the time, little to no targets are known. In Lalaouna et al. (2015), we proposed a new technology to uncover sRNAs targetome, which is based on the MS2-affinity purification (MAPS). We were able to prove its efficiency by applying it on well-characterized sRNAs of Escherichia colt. Thereafter, we adapted the procedure to other kind of RNA (mRNAs and tRNA-derived RNA fragments) and bacteria (pathogenic or Gram-positive strains). Here, we clearly report all improvements and adjustments made to MAPS technology since it was originally reported. (C) 2016 Elsevier Inc. All rights reserved.
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