期刊
ANALYST
卷 144, 期 4, 页码 1253-1259出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8an02139b
关键词
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资金
- National Natural Science Foundation of China [81601856]
- Third Batch of Young Backbone Teachers Funding Program in Colleges and Universities of Chongqing City [53]
- Funds for Young Science and Technology Talent Cultivation Plan of Chongqing City [cstc2014kjrc-qnrc00004]
Lipopolysaccharides (LPS), known as endotoxins, can cause a strong inflammatory response and lead to multiple organ failure in severe cases. This work reports a simple label-free voltammetric aptasensor for highly sensitive determination of LPS using a polyethyleneimine (PEI) functionalized reduced graphene oxide (rGO) and molybdenum disulfide (MoS2) composite (PEI-rGO-MoS2) as a new nanocarrier for electroactive toluidine blue (TB). The PEI-rGO-MoS2 nanocomposite with high electrical conductivity and large specific surface area can greatly increase the loading of TB and facilitate electron transfer from TB to an electrode. Then gold nanoparticles (AuNPs) were utilized to immobilize a thiolated LPS binding aptamer (LBA), which not only exhibited excellent biocompatibility, but also significantly amplified the electrochemical signal of TB. The proposed aptasensor exhibited high sensitivity for LPS and showed a wide linear range from 5.0 x 10(-5) ng mL(-1) to 2.0 x 10(2) ng mL(-1) with a low limit of detection (LOD) of 3.01 x 10(-5) ng mL(-1), which overcame the shortcomings of traditional detection methods and achieved fast and accurate detection of LPS. Moreover, it exhibited excellent recovery and specificity upon spiking LPS in serum samples, indicating that this method has promising application in the field of trace analysis of LPS in clinical detection.
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