Development of peroxidase enzyme immobilized magnetic nanoparticles for bioremediation of textile wastewater dye

Peroxidase enzyme plays a major role in the degradation of phenolic compounds such as azo dyes. This enzyme was isolated and purified from the bioremediation products of textile wastewater containing direct green azo dye in a UFC/CSA prototype bioreactor. To improve the enzyme stability, efficiency and recyclability, peroxidase was immobilized on Fe3O4 magnetic nanoparticles produced and modified by co-precipitation using glutaraldehyde. The immobilized enzyme was characterized by transmission electron microscopy analysis and examined against different pH as well as storage time and temperatures. The MNPs-immobilized peroxidise was remarkable stable towards temperature and pH perturbations, compared to the free enzyme form. Moreover, the immobilized peroxidase maintained its full activity upon storage at 4 and 25 degrees C for 90 days, and upon recycling for up to 100 cycles. Finally, the MNPs-immobilized peroxidase was successfully employed to decolorize dyes of different chemical natures in a lab scale bioreactor. Hence, we believe that the immobilized peroxidase on magnetic nanoparticles reported in this work can be potentially used in various industrial and environmental applications.