期刊
HORTICULTURAL PLANT JOURNAL
卷 5, 期 1, 页码 31-38出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.hpj.2018.10.002
关键词
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资金
- National Natural Science Foundation of China [31272205, 31672196]
- Fundamental Research Funds for Central Non-profit Scientific Institutions
- Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences
Gene function analysis is challenging for Lilium due to the lack of an efficient method for stable genetic transformation. Virus-induced gene silencing (VIGS) is an attractive tool for determining gene function in plants. This study reported that Tobacco rattle virus (TRV)-based VIGS of a PHYTOENE DESATURASE (PDS) ortholog (LhPDS) can be achieved in Lilium x formolongi seedlings, with a survival rate of 92%, using the inoculation method of rubbing plus injection. Compared with untreated and mock-treated seedlings, the photobleached leaf phenotype of silenced plants significantly correlates with down-regulation of endogenous LhPDS (P <= 0.05). In addition, the silencing phenomenon can be observed in the growing points of plants, indicating that systemic viral infection was achieved using the protocol. The results indicate that TRV-based VIGS can be used to characterize gene function in Lilium x formolongi. This work lays the foundation for gene function analysis and molecular breeding in Lilium spp.
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