期刊
DRUG RESEARCH
卷 69, 期 3, 页码 151-158出版社
GEORG THIEME VERLAG KG
DOI: 10.1055/a-0654-5042
关键词
Ocimum; sickness behaviour; lipopolysaccharides; flavonoids
PurposeOcimum gratissimum L. leaves has been traditionally used for management of febrile illnesses and symptoms typified of sickness behavior. In this work we investigated the modulatory effect of flavonoid-rich fraction of O. gratissimum leaves (EAFO g ) on sickness behavior, inflammatory and oxidative stress responses in LPS-challenged mice. MethodO. gratissimum leaf was first extracted with n-hexane, chloroform and methanol, and EAFO g was obtained by ethylacetate partitioning of a sequentially resultant methanol extract. The effect of EAFO g (25-100mg/kg) on acute LPS-induced neurobehavioral impairment in an open field test (OFT) and depressive-like behavior in forced swimming test (FST) was investigated. Serum nitrite and TNF-, as well as myeloperoxidase (MPO), malondialdehyde (MDA), and reduced glutathione (GSH) levels were determined in liver and brain tissues. Result EAFO g prevented the reduction in locomotor and rearing activity in OFT and the increase in immobility time in FST. The fraction significantly attenuated the elevation of serum TNF- and nitrite levels. EAFOg reversed LPS-induced increase in MDA, MPO, and nitrite levels and attenuated GSH depletion in liver and brain tissues of mice. Conclusion Flavonoid-rich fraction of O. gratissimum leaf demonstrated significant modulation of LPS-induced sickness behavior, inflammatory and oxidative stress response in mice. This suggests an important therapeutic strategy in slowing down LPS-mediated hepatic and neuronal disease processes.
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