期刊
TOXICOLOGY RESEARCH
卷 8, 期 2, 页码 297-308出版社
OXFORD UNIV PRESS
DOI: 10.1039/c8tx00323h
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- University Grants Commission (UGC), Government of India, New Delhi, India [FIP/12th plan/KLMG034 TF13]
In this work, silver nanoparticles (AgNP-DTa) were prepared using an aqueous seed extract of D. trifoliata. The importance of the present piece of work is viewed specially with respect to ascertaining the potential of a widely distributed under-utilized mangrove associated plant, Derris trifoliata (DT), as medicine. The as-prepared AgNP-DTa were well dispersed and stabilised in aqueous solution through biological ligands extracted from the seeds of DT. The functional groups present in the bio-ligands of DT act as reducing and stabilising agents in the formation of nanoparticles. Besides, in the present work, sunlight could induce and catalyse the reduction process of Ag+ to its corresponding silver atoms of nanoscale dimensions. The size of AgNP-DTa decreased with an increase in the duration of sunlight irradiation. Bio-augmented nanoparticles were characterized by UV-vis spectroscopy, XRD, HR-TEM, DLS, AFM and photoluminescence measurements. Preliminary phytochemical studies and FTIR analysis confirmed the presence of secondary metabolites with hydroxyl, amine and carbonyl groups as reducing/capping agents. AgNP-DTa demonstrated high DPPH scavenging activity with an IC50 value of 8.25 mu g ml(-1). Greater antioxidant activity of AgNP-DTa was also confirmed from total antioxidant capacity (TAC) assay where it was found that the reducing power of 1 g of AgNP-DTa is almost equivalent to that of 1.3 g of Trolox. In addition, highly stable AgNP-DTa showed antibacterial activities against Gram positive and Gram negative bacteria. The as-prepared AgNP-DTa were observed to inhibit the growth of Klebsiella pneumonia, Staphylococcus aureus and Escherichia coli and no clear zone was obtained for Pseudomonas aeruginosa. With reference to the anti-proliferative activities, AgNP-DTa exhibited moderate activity on A549 lung cancer cell lines with a median effective concentration of 86.23 +/- 0.22 mu g ml(-1).
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