期刊
MEDICINAL RESEARCH REVIEWS
卷 38, 期 1, 页码 229-260出版社
WILEY
DOI: 10.1002/med.21439
关键词
genome engineering; genome modification; synthetic biology; antibiotics
资金
- National Natural Science Foundation of China [31520103902, 31170070, 31670086, 31670072]
- 973 program of Ministry of Science and Technology [2013CB734003]
- Hubei Province's Outstanding Medical Academic Leader Program
- Young One Thousand Talent program of China
Antibiotic production is often governed by large gene clusters composed of genes related to antibiotic scaffold synthesis, tailoring, regulation, and resistance. With the expansion of genome sequencing, a considerable number of antibiotic gene clusters has been isolated and characterized. The emerging genome engineering techniques make it possible towards more efficient engineering of antibiotics. In addition to genomic editing, multiple synthetic biology approaches have been developed for the exploration and improvement of antibiotic natural products. Here, we review the progress in the development of these genome editing techniques used to engineer new antibiotics, focusing on three aspects of genome engineering: direct cloning of large genomic fragments, genome engineering of gene clusters, and regulation of gene cluster expression. This review will not only summarize the current uses of genomic engineering techniques for cloning and assembly of antibiotic gene clusters or for altering antibiotic synthetic pathways but will also provide perspectives on the future directions of rebuilding biological systems for the design of novel antibiotics.
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