期刊
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
卷 114, 期 -, 页码 190-197出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ecoenv.2015.01.025
关键词
Lipid peroxidation; DNA methylation; Environmental complex mixture; River water; Oreochromis niloticus
资金
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq - Brazil)
- Laboratorio de Toxicologia Ambiental (LABTOX/UFSC)
In this study, we investigated the effects of oxidative stress and hypermethylation through lipid peroxidation and DNA methylation, respectively, in erythrocytes of Oreochromis niloticus exposed to environmental complex mixture of water from Cubatao do Sul River throughout the year. This river is the source of drinking water for the region of Florianopolis, the capital of Santa Catarina State, Brazil. Lipid peroxidation was quantified by the rate of malondialdehyde (MDA) formation, and DNA methylation was quantified by the rate of 5-methyldeoxycytosine (m(5)dC) formation. In all studied sites, the river water samples caused metabolic changes in O. niloticus. MDA formation rates were significantly different when compared to the negative control (except for samples from Site 1 during spring 2010, summer 2011 and fall 2011). All samples (except Site 1, spring 2010) induced increases in the m(5)dC formation rates, and at the end of the study, the values were near the values found in the positive control (potassium dichromate 2.5 mg/L). The results showed that samples of environmental complex mixtures of water from Cubatao do Sul River are capable of inducing high levels of oxidative damage and hypermethylation in O. niloticus. (C) 2015 Elsevier Inc. All rights reserved.
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