4.2 Article

Pilot Study of Vaginal Microbiome Using QIIME 2™ in Women With Gynecologic Cancer Before and After Radiation Therapy

期刊

ONCOLOGY NURSING FORUM
卷 46, 期 2, 页码 E48-E59

出版社

ONCOLOGY NURSING SOC
DOI: 10.1188/19.ONF.E48-E59

关键词

vaginal microbiome; 16S rRNA gene; gynecologic cancer; radiation therapy

资金

  1. Emory Integrated Genomics Core Shared Resource of Winship Cancer Institute of Emory University
  2. National Institutes of Health/National Cancer Institute [P30CA138292]
  3. Friends of Winship Fashion Research Scholar Award from the Winship Cancer Institute

向作者/读者索取更多资源

OBJECTIVES: To characterize the vaginal microbiome using QIIME 2 (TM) (Quantitative Insights Into Microbial Ecology 2) in women with gynecologic cancer. SAMPLE & SETTING: 19 women with gynecologic cancer before and after radiation therapy at a comprehensive cancer center in Atlanta, Georgia. METHODS & VARIABLES: This pilot study analyzed vaginal microbiome communities using a microbiome analysis pipeline, beginning with 16S rRNA gene sequencing and processing through use of a bioinformatics pipeline to downstream microbial statistical analysis. RESULTS: The findings showed the methods to be robust, and most women with gynecologic cancer showed depletion of Lactobacillus. Compared to those pre-radiation therapy. women post-radiation therapy showed higher abundances of Mobiluncus, Atopobium, and Prevotella but lower abundances of Lactobacillus, Gardnerella, and Peptostreptococcus, which are associated with bacterial vaginosis. IMPLICATIONS FOR NURSING: This study presents the fundamentals of human microbiome data collection and analysis methods to inform nursing science. Assessing the vaginal microbiome provides a potential pathway to develop interventions to ameliorate dysbiosis of the vaginal microbiome.

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