期刊
ISCIENCE
卷 12, 期 -, 页码 141-+出版社
CELL PRESS
DOI: 10.1016/j.isci.2018.12.032
关键词
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资金
- Israel Science Foundation [372/17]
- US Army Life Sciences Division [LS67993]
- US-Israel Binational Science Foundation [2013060]
- Negev Scholarship for Excellent Graduate Students
- Harbor Foundation
- Office Of Information & Resource Mgmt [2013060] Funding Source: National Science Foundation
Unlike the nuclear genome, the mammalian mitochondrial genome (mtDNA) is thought to be coated solely by mitochondrial transcription factor A (TFAM), whose binding sequence preferences are debated. Therefore, higher-order mtDNA organization is considered much less regulated than both the bacterial nucleoid and the nuclear chromatin. However, our recently identified conserved DNase footprinting pattern in human mtDNA, which co-localizes with regulatory elements and responds to physiological conditions, likely reflects a structured higher-order mtDNA organization. We hypothesized that this pattern emerges during embryogenesis. To test this hypothesis, we analyzed assay for transposase-accessible chromatin sequencing (ATAC-seq) results collected during the course of mouse and human early embryogenesis. Our results reveal, for the first time, a gradual and dynamic emergence of the adult mtDNA footprinting pattern during embryogenesis of both mammals. Taken together, our findings suggest that the structured adult chromatin-like mtDNA organization is gradually formed during mammalian embryogenesis.
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