期刊
ANALYST
卷 144, 期 5, 页码 1704-1710出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c8an02196a
关键词
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资金
- Shandong Provincial Natural Science Foundation [ZR201709250117, ZR2018ZC0230]
- National Natural Science Foundation of China [51473067, 21675064]
- 111 Project of International Corporation on Advanced Cement-based Materials [D17001]
- Young Taishan Scholars Program of Shandong Province, China [TSQN 20161036]
The superoxide anion (O-2(-)) plays a crucial role in several physiological processes and many human diseases. Developing new methods for O-2(-) detection in biological systems is very important. A FRET-based two-photon (TP) fluorescent probe with a ratiometric signal, TFR-O, was developed. A naphthalene derivative based TP fluorescent group was selected as the energy donor group, and a rhodol fluorescent group was chosen as the energy acceptor; the trifluoromethanesulfonate group was chosen as the recognition moiety. After reacting with O-2(-), the recognition moiety was removed and the fluorophore was released, leading to a fluorescence intensity decrease at the wavelength of 425 nm and a significant enhancement of the fluorescence intensity at 550 nm. The fluorescence intensity ratio between 550 and 425 nm (I-550/I-425) varied from 0.15 to 6.72, with the O-2(-) concentration increasing from 0 to 50 M. The detection limit of the TFR-O was 83 nM. Moreover, TFR-O was applied for detecting and imaging O-2(-) in cells and liver tissues.
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