期刊
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
卷 7, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fbioe.2019.00060
关键词
bioelectrochemical systems; biofilm thickness; flow cytometry; Geobacter sulfurreducens; microbial electrolysis cell; mixed culture; planktonic; Shewanella oneidensis
资金
- NTH-Research Unit ElektroBak-Innovative materials and concepts for microbial electrochemical systems within the Braunschweig Integrated Centre of Systems Biology (BRICS) at the Technische Universitat Braunschweig, Germany
- German Research Foundation
- Technische Universitat Braunschweig
This work aims to investigate the long-term behavior of interactions of electrochemically active bacteria in bioelectrochemical systems. The electrochemical performance and biofilm characteristics of pure cultures of Geobacter sulfurreducens and Shewanella oneidensis are being compared to a defined mixed culture of both organisms. While S. oneidensis pure cultures did not form cohesive and stable biofilms on graphite anodes and only yielded 0.034 +/- 0.011 mA/cm(2) as maximum current density by feeding of each 5 mM lactate and acetate, G. sulfurreducens pure cultures formed 69 mu m thick, area-wide biofilms with 10 mM acetate as initial substrate concentration and yielded a current of 0.39 +/- 0.09 mA/cm(2). Compared to the latter, a defined mixed culture of both species was able to yield 38% higher maximum current densities of 0.54 +/- 0.07 mA/cm(2) with each 5 mM lactate and acetate. This increase in current density was associated with a likewise increased thickness of the anodic biofilm to approximately 93 mu m. It was further investigated whether a sessile incorporation of S. oneidensis into the mixed culture biofilm, which has been reported previously for short-term experiments, is long-term stable. The results demonstrate that S. oneidensis was not stably incorporated into the biofilm; rather, the planktonic presence of S. oneidensis has a positive effect on the biofilm growth of G. sulfurreducens and thus on current production.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据