期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 78, 期 -, 页码 949-959出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2017.03.300
关键词
ZnO nanoparticles; MTCP; Apoptosis; Autophagy; Calcium; Breast cancer
资金
- Iran National Science Foundation (INSF) [93002224]
- Research to Prevent Blindness to the Department of Ophthalmology and Visual Sciences
- Retina Research Foundation [P30 EY016665, P30 CA014520, EPA 83573701, EY022883]
Zinc oxide nanoparticles are very toxic but their agglomeration reduces their lethal cytotoxic effects. Here we tested the hypothesis that conjugation of ZnO nanoparticles via Meso-Tetra (4-Carboxyphenyl) Porphyrin (MTCP) could provide electrostatic or steric stabilization of ZnO nanoparticles and increase their cytotoxic effects. The cytotoxicity and cell death induction were assessed using two human breast adenocarcinoma cell lines (MCF-7 and MDA-MB-468). The MIT results indicated that the toxicity of ZnO nanoparticles was significantly increased upon MTCP conjugation. Annexin/PI and real time RT-PCR results demonstrated that the ZnO-MTCP nanoparticles induced cell death via different non-canonical pathways that are under ca(2+) control. Calcium signaling could regulate lysosomal dependent apoptosis and death autophagy, and killing of the two selected types of breast cancer cells. (C) 2017 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据