期刊
MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
卷 77, 期 -, 页码 1111-1116出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2017.04.029
关键词
Gold nanoclusters; Biotemplating; Photoluminescence; Biomimetics; Cellular imaging
资金
- Institute of Materials Research and Engineering, Agency of Science, Technology and Research (A*STAR), under Boinspired Approaches to Biomimetic Materials program [IMRE/00-1P1400]
Bioluminescence has been widely recognized as a powerful imaging tool for biological investigations. Synthesis of molecular lanterns that mimic bioluminescence in nature is of great interest. Herein we report a synthesis of molecular lantern by utilizing the catalytic properties of ultrasmall (<2 nm) gold nanoclusters (AuNC), which is inspired by the enzymatic light-up of luciferin in the biological system. Small molecules such as hydroquinone and Cys-Gly peptides are used to direct the synthesis of AuNC via a biotemplating approach, while the in-situ formation of AuNC simultaneously catalyze the formation of luciferin-like, brightly fluorescent dye (lambda(em) = 520 nm, QY = 020). The as-formed dye species deposits on the surface of AuNC and prevents its agglomeration, thus forming a dye-decorated AuNC core-shell structure (AuNC@dye). The formation mechanism of the AuNC@dye composite material has been systematically studied using different characterization techniques including TEM, ultraviolet visible spectroscopy, and photoluminescence measurements. The biocompatibility of as-formed AuNC@dye is verified by cellular uptake experiments, and it has been demonstrated as a good imaging probe by using Hela cells as a model. (C) 2017 Elsevier B.V. All rights reserved.
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