期刊
ACS OMEGA
卷 4, 期 3, 页码 4814-4824出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsomega.8b02467
关键词
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资金
- DST-INSPIRE Fellowship Scheme, New Delhi, India [DST/INSPIRE-Fellowship/2012/251]
- DST
- DST-SERB
A one-pot greener methodology has been adopted for the synthesis of a simple 4H-chromene core-based fluorescent tag of (S)-2-amino-4-(anthracen-9-yl)-7-hydroxy-4H-chromene-3-carbonitrile (AHC), and its structure has been analyzed using NMR spectroscopy. The physicochemical properties of AHC were well-studied by UV-vis and fluorescent spectroscopy techniques. As a result of excellent emitting property (phi approximate to 0.75), it has been coupled with anti-AH through amide linkage, and the AHC-tagged anti-AH has been used as an immunoassay for the selective detection of Aeromonas hydrophila in the presence of interfering pathogens. Under optimized conditions, immunosensors could successfully quantify A. hydrophila from 4 to 736 CFU/mL, and the LOD was 2 CFU/mL. Saliently, the immunoassay has been successfully demonstrated for the analysis of A. hydrophila in the organs of Oreochromis mossambicus fingerlings, and results have shown a very good agreement with our optimized neat AH fluorimetric titration results.
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