期刊
MASS SPECTROMETRY REVIEWS
卷 37, 期 5, 页码 583-606出版社
WILEY
DOI: 10.1002/mas.21550
关键词
disease research; isobaric labeling; immunodepletion; peptide fractionation; plasma; protein biomarkers
类别
资金
- National Technology Agency of Finland (Finland Distinguished Professor Programme) [40398/11]
- Juvenile Diabetes Research Foundation (JDRF) [17-2011-586]
- Academy of Finland (Centre of Excellence in Molecular Systems Immunology and Physiology Research) [250114]
- Biocenter Finland (Turku Proteomics Facility)
Over the past decade, chemical labeling with isobaric tandem mass tags, such as isobaric tags for relative and absolute quantification reagents (iTRAQ) and tandem mass tag (TMT) reagents, has been employed in a wide range of different clinically orientated serum and plasma proteomics studies. In this review the scope of these works is presented with attention to the areas of research, methods employed and performance limitations. These applications have covered a wide range of diseases, disorders and infections, and have implemented a variety of different preparative and mass spectrometric approaches. In contrast to earlier works, which struggled to quantify more than a few hundred proteins, increasingly these studies have provided deeper insight into the plasma proteome extending the numbers of quantified proteins to over a thousand.
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