期刊
GENOME BIOLOGY
卷 20, 期 -, 页码 -出版社
BMC
DOI: 10.1186/s13059-019-1671-x
关键词
RNA-seq; Transcriptomics; Gene expression; qPCR; Barcoding
资金
- Commission for Technology and Innovation [17945.2 PFLS-LS]
- Swiss National Science Foundation [31003A_162735, IZLIZ3_156815]
- Precision Health & related Technologies Initiative [PHRT-502]
- Swiss National Science Foundation (SNF) [31003A_162735, IZLIZ3_156815] Funding Source: Swiss National Science Foundation (SNF)
Despite its widespread use, RNA-seq is still too laborious and expensive to replace RT-qPCR as the default gene expression analysis method. We present a novel approach, BRB-seq, which uses early multiplexing to produce 3 cDNA libraries for dozens of samples, requiring just 2hours of hands-on time. BRB-seq has a comparable performance to the standard TruSeq approach while showing greater tolerance for lower RNA quality and being up to 25 times cheaper. We anticipate that BRB-seq will transform basic laboratory practice given its capacity to generate genome-wide transcriptomic data at a similar cost as profiling four genes using RT-qPCR.
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