Extraction and characterization of acid soluble collagen from fish waste: Development of collagen-chitosan blend as food packaging film

Skin of Centrolophus niger (black ruff) commonly referred as Medusa, is used for the extraction of Acid Soluble Collagen. This is the first report of collagen extraction from skin of black ruff where lactic acid with 45% yield proved to be efficient solvent. SDS-PAGE, UV visible absorbance, FT-IR and SEM analysis confirmed that extracted collagen is native type I. Extracted collagen showed emulsifying activity index as 20.24 m(2) g(-1) and emulsion stability index of 15.86 min. Collagen was used for the preparation of collagen-chitosan film which could be used for food packaging purpose. Film incorporated with 5% pomegranate peel extract declined solubility in water remarkably and showed antibacterial effect against food borne pathogens like Bacillus saprophyticus LNB 333F5, Bacillus subtilis NCIM 2635, Salmonella typhi NCIM 2501 and Escherichia coli NCIM 2832. Thus, present study deals with extraction and characterization of collagen from fish waste and its application in the development of antibacterial active food packaging film.