4.3 Article

Anti-Candida activity of beneficial vaginal lactobacilli in in vitro assays and in a murine experimental model

期刊

FEMS YEAST RESEARCH
卷 19, 期 2, 页码 -

出版社

OXFORD UNIV PRESS
DOI: 10.1093/femsyr/foz008

关键词

Candida spp.; beneficial lactobacilli; murine model; vulvovaginal candidiasis; in vitro and in vivo studies; anti-Candida activity

资金

  1. Consejo Nacional de Investigaciones Cientificas y Tecnicas, Argentina (CONICET) [PIP 545]
  2. Agencia Nacional de Promocion Cientifica y Tecnologica (ANPCyT) [PICT 2017-4324]

向作者/读者索取更多资源

Vulvovaginal candidiasis (WC) is one of the most frequent infections affecting women worldwide. Healthy vaginal microbiota is dominated by lactobacilli, which form a strong defense line against pathogens. In this work, in vitro antimicrobial properties of thirty vaginal Lactobacillus strains were evaluated against eleven Candida vaginal clinical isolates, employing three different methods. Also, the effect of intravaginal (i.va.) administrations (preventive, therapeutic and preventive-therapeutic) of L. reuteri CRL1324 or L. rhamnosus CRL1332 strains against the i.va. challenge with Candida albicans C2 (C.a.) was evaluated in a murine experimental model. From the results of agar overlay and liquid medium assays the selected lactobacilli strains have shown to inhibit the growth of at least one Candida strain. The inhibition was mainly due to the effect of organic acids. Anti-Candida activity was not evidenced in the agar plate diffusion method. In the experimental murine model, only preventive-therapeutic administration of both lactobacilli was able to significantly reduce viable C.a. numbers recovered in vaginal washes and the leukocyte influx induced by the fungi. In conclusion, lactobacilli exhibited in vitro and in vivo antimicrobial effects on Candida, suggesting that they could be promising candidates for protection against VVC.

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