期刊
MARINE DRUGS
卷 15, 期 11, 页码 -出版社
MDPI AG
DOI: 10.3390/md15110332
关键词
chitooligosaccharides; deacetylation; bromocresol green; acid-base titration; first-order derivative UV spectra; H-1 NMR
资金
- Industry University Research Collaborative Innovation Major Projects of Guangzhou Science Technology Innovation Commission, China [201604020164]
- Science and Technology Planning Project of Yunfu, Guangdong, China [201702-9]
- Science and Technology Planning Project of Guangdong, China [2013B021100018]
- National Science Foundation of China [81173107]
The methods for determination of chitosan content recommended in the Chinese Pharmacopoeia and the European Pharmacopoeia are not applicable for evaluation of the extent of deacetylation (deacetylation degree, DD) in chitooligosaccharides (COS). This study explores two different methods for assessment of DD in COS having relatively high and low molecular weights: an acid-base titration with bromocresol green indicator and a first order derivative UV spectrophotometric method for assessment of DD in COS. The accuracy of both methods as a function of molecular weight was also investigated and compared to results obtained using H-1 NMR spectroscopy. Our study demonstrates two simple, fast, widely adaptable, highly precise, accurate, and inexpensive methods for the effective determination of DD in COS, which have the potential for widespread commercial applications in developing country.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据