期刊
MARINE BIOTECHNOLOGY
卷 19, 期 2, 页码 136-146出版社
SPRINGER
DOI: 10.1007/s10126-017-9735-y
关键词
Anti-proliferation; Immunomodulation; Exo-polysaccharide; Microalgae; Thraustochytriidae
资金
- KRIBB Research Initiative Program [KGM2211531]
- National Research Foundation of Korea (NRF) - Ministry of Education, Science and Technology (MEST) of the Republic of Korea [2014R1A1A2055295]
- Priority Research Centers Program through NRF - Ministry of Education, Science and Technology [2015R1A6A1A04020885]
- National Research Council of Science & Technology (NST), Republic of Korea [KGM5171711] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- National Research Foundation of Korea [2014R1A1A2055295, 2015R1A6A1A04020885, 21A20132112253] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Marine microalgal exopolysaccharides (EPSs) have drawn great attention due to their biotechnological potentials such as anti-viral, anti-oxidant, anti-lipidemic, anti-proliferative, and immunomodulatory activities, etc. In the present study, the EPS derived from microalgae Thraustochytriidae sp.-derived mutant GA was investigated for its anti-proliferation and immunomodulation. Anti-cancer efficacy of the microalgal EPS was examined for the alterations in cell proliferation and cell cycle-related gene expression that occur in three types of human cancer cell lines, BG-1 ovarian, MCF-7 breast, and SW-620 colon cancer cell lines, by its treatment. Alterations in immunoreactivity by the microalgal EPS were examined by measuring its influence on the growth of T and B lymphocytes and cytokine production of T cells. In cell viability assay, the microalgal EPS inhibited cancer cell growth at the lowest concentration of 10(-11) dilution and in a dose-responsive manner within the range of dilution of 10(-11)similar to 10(-3). In addition, the protein expression of cell cycle progression genes such as cyclin D1 and E in these cancer cell lines was significantly reduced by the microalgal EPS in a dose- and a time-dependant manner. In cell proliferation assay using T and B cells, the microalgal EPS induced B cell proliferation even at the lowest dilution of 10(-11), but not T cells. In cytokine assay, the microalgal EPS decreased the formation of IL-6 and INF-gamma at 10(-3) dilution compared to the control and had no significant effects on TNF-alpha. Collectively, these findings suggest that the EPS derived from microalgae Thraustochytriidae sp. GA has an anti-proliferative activity against cancer cells and an immunomodulatory effect by having an influence on B cell proliferation and cytokine secretion of T cells.
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