期刊
MALARIA JOURNAL
卷 16, 期 -, 页码 -出版社
BIOMED CENTRAL LTD
DOI: 10.1186/s12936-017-2061-z
关键词
Malaria; Deletion; RDT; Mozambique; Pfhrp2
资金
- Bill and Melinda Gates Foundation [OPP111526]
- La Caixa Foundation [OPP111526]
- Instituto de Salud Carlos III [Fondo Europeo de Desarrollo Regional (FEDER)] [PI13/01478, CES10/021-I3SNS]
- Overseas Postdoctoral Fellowship programme by Science and Engineering Research Board, Department of Science & Technology, Government of India [SB/OS/PDF-043/2015-16]
- Spanish Agency for International Cooperation (AECI)
Background: Malaria programmes use Plasmodium falciparum histidine-rich protein-2 (PfHRP2) based rapid diagnostic tests (RDTs) for malaria diagnosis. The deletion of this target antigen could potentially lead to misdiagnosis, delayed treatment and continuation of active transmission. Methods: Plasmodium falciparum isolates (n = 1162) collected in Southern Mozambique were assessed by RDTs, microscopy and/or 18SrRNA qPCR. pfhrp2 and pfhrp3 deletions were investigated in isolates from individuals who were negative by RDT but positive by microscopy and/or qPCR (n = 69) using gene-specific PCRs, with kelch13 PCR as the parasite DNA control. Results: Lack of pfhrp2 PCR amplification was observed in one of the 69 isolates subjected to molecular analysis [ 1.45% (95% CI 0.3-7.8%)]. Conclusions: The low prevalence of pfhrp2 deletions suggests that RDTs will detect the vast majority of the P.falciparum infections. Nevertheless, active surveillance for changing deletion frequencies is required.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据