期刊
NATURE PLANTS
卷 5, 期 5, 页码 480-485出版社
NATURE PORTFOLIO
DOI: 10.1038/s41477-019-0405-0
关键词
-
资金
- National Key Research and Development Program of China [2016YFD0101804]
- National Natural Science Foundation of China [31788103, 31471785, 31872933, 31570369]
- Chinese Academy of Sciences [QYZDY-SSW-SMC030, KFJ-STS-ZDTP-024]
- Beijing Municipal Science and Technology [D171100007717001, Z171100001517001]
Developing herbicide-tolerant varieties by genome editing holds great promise for addressing the worsening weed problems in wheat cultivation(1). Here, we generated transgene-free wheat germplasms harbouring herbicide tolerance mutations that confer tolerance to sulfonylurea-, imidazolinone- and aryloxyphenoxy propionate-type herbicides by base editing the acetolactate synthase (ALS) and acetyl-coenzyme A carboxylase genes. These stackable herbicide tolerance traits provide a potentially powerful tool for weed management. In addition, we found that base editing at the wheat ALS Pro-174 codon (TaALS-P174) endowed wheat with sufficient resistance to nicosulfuron herbicide in MS growth medium to allow selection. When the TaALS-P174 editor was coupled with editors for other targets of interest, co-editing occurred in the nicosulfuron-resistant plants, and selection for resistance in growth medium enriched the frequency of coupled targets by several-fold. This selectable co-editing system has the potential to greatly bolster adoption of base editing for crop improvement applications.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据