期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 83, 期 -, 页码 283-291出版社
ELSEVIER
DOI: 10.1016/j.lwt.2017.05.030
关键词
Maize; Aflatoxin; Aspergillus flavus; aflR; Environment
资金
- Spanish Ministry of Education, Science and Sport [AP 2012-5457]
- Spanish Ministry of Economy and Competitiveness [BES-2014-069484, IJCI-2014-20666]
- Spanish Interministerial Committee on Science and Technology [CSD2007-00016, AGL 2013-45729-P]
- Government of Extremadura
- FEDER [GR15108]
Maize is prone to infection by Aspergillus flavus, which can contaminate the product with aflatoxins. The objective of this study was to examine the impact that interactions between water activity (a(w)) and temperature may have on growth, the expression of a biosynthetic regulatory gene (aflR) and aflatoxin B-1 (AFB(1)) production by a strain of A. flavus on a maize-based medium. Results showed that there were some differences between lag phases and growth rates of A. flavus. The optimum growth rate for A. flavus was at 30 degrees C and 0.99 a(w). No growth occurred at 0.90 a(w) and 20 degrees C. Both temperature and a(w) had an influence on the relative aflR gene expression and AFB(1) production by A. flavus; however, the results for AFB(1) production were not consistent with the effects on gene expression or growth. These findings provide data that are useful to better understand the conditions which represent higher risks from AFB(1) production. However, the aflR expression was not a good indicator of AFB(1) production alone. Thus, further molecular studies of other AF-related genes should be done. These results are discussed in the context of harvesting and storage of maize and the prevailing environmental conditions to minimise AFB(1) contamination. (C) 2017 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据