期刊
LWT-FOOD SCIENCE AND TECHNOLOGY
卷 76, 期 -, 页码 198-202出版社
ELSEVIER
DOI: 10.1016/j.lwt.2016.07.037
关键词
Photoinactivation; Curcumin; LED; Bacteria
The present study evaluated the efficacy of photodynamic inactivation (PDI) of foodborne and spoilage bacteria using curcumin and a blue light emitting diode (LED). Curcumin at 75 mu M was used to photo-irradiate Staphylococcus aureus ATCC 25923, Aeromonas hydrophila ATCC 7966, Salmonella Typhimurium ATCC 14028, Escherichia coli ATCC 25922 and Pseudomonas aeruginosa ATCC 27853 at light doses of 139 J/cm(2), 278 J/cm(2) and 417 J/cm(2). The cytotoxicity of curcumin in VERO cells was evaluated in similar conditions to bacterial photoinactivation assay, and the percentage of cell destruction was congruent to 13 +/- 0.05%, for all light doses. Curcumin-mediated PDI of S. aureus induced a significant reduction of approximately 3.50 log CFU/ml at 139 J/cm(2) and 278 J/cm(2). Full inactivation was observed at 417 J/cm(2). Among Gram negative bacteria, P. aeruginosa was the least susceptible to PDI, which counts were not significantly reduced. A significant reduction in E. coli counts was observed at 278 J/cm(2), and no viable cells were detected after light exposure at 417 J/cm(2). When photo -irradiated with curcumin at 278 J/cm(2) and 417 J/ cm(2), A. hydrophila was completely eradicated, while a significant decrease (3.331og CFU/ml) was observed in bacterial counts at 139 J/cm(2). Curcumin in combination with a blue LED is a potential candidate for PDI against Gram positive and Gram negative bacteria. (C) 2016 Elsevier Ltd. All rights reserved.
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