期刊
RSC ADVANCES
卷 9, 期 19, 页码 10832-10841出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c9ra01764j
关键词
-
资金
- Japan Society for the Promotion of Science [15K06474]
- Japan Science and Technology Agency [AS282I011e]
- Grants-in-Aid for Scientific Research [15K06474] Funding Source: KAKEN
High catalytic activities of enzymes are necessary for enzyme immobilising technology for the development of glucose sensors. The aim of this study is to synthesise two types of poly(l-lysine)-containing calcium phosphate particles (pLys-HAp) and to achieve the immobilisation of glucose oxidase (GOX) on them. The oxidation activity of GOX immobilised on these particles was more than 80% compared to that of native GOX (considered to be 100%). Additionally, the relative activity of GOX immobilised on poly-epsilon-lysine-containing HAp (epsilon-pLys-HAp) remained approximately 70% after ten cycles. Moreover, glucose detection was able to be performed in the linear range of 4-400 M using GOX immobilised on pLys-HAp composites. In the direct electrochemistry measurement using the cyclic voltammetry (CV) method, a glassy carbon electrode (GCE) modified by epsilon-pLys-HAp was a good enzyme electrode and can be used for glucose detection with high sensitivity. From these results, poly(l-lysine)-containing HAp composites can be expected to be enzyme immobilisation agents with high stability and biosensors with high sensitivity.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据