期刊
LUMINESCENCE
卷 33, 期 1, 页码 190-195出版社
WILEY
DOI: 10.1002/bio.3392
关键词
carbon nitride nanosheet; catalytic hairpin assembly; intracellular signal amplification; microRNA; nanoprobe
资金
- National Natural Science Foundation of China [21665026]
- Natural Science Foundation of Guangxi Province [2016GXNSFBA380240]
In this study, an ultrasensitive fluorescence turn-on assay for in situ sensing of intracellular microRNA (miRNA) was developed utilizing a carbon nitride nanosheet (CNNS) and a catalytic hairpin assembly (CHA). The CHA showed favourable signal amplification for low-level biomarkers, and CNNS was an excellent candidate as a fluorescence quencher and gene vector. Moreover, the hairpin DNA of CHA could be adsorbed onto the surface of CNNS. An enzyme-free fluorescence biosensor for ultrasensitive sensing of intracellular miRNA in cells based on CHA and CNNS was designed. When faced with target miRNA, the fluorescence was recovered due to the miRNA, which could trigger cycling of CHA circuits, leading to the production of a marked enhanced fluorescence signal. Compared with traditional methods, the proposed method is convenient, with low cytotoxicity, and high specificity and ultrasensitivity. It has promising potential for detection low-level biomarkers.
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