期刊
INDIAN JOURNAL OF DERMATOLOGY VENEREOLOGY & LEPROLOGY
卷 85, 期 3, 页码 258-265出版社
WOLTERS KLUWER MEDKNOW PUBLICATIONS
DOI: 10.4103/ijdvl.IJDVL_275_17
关键词
Melanocytes; spheroid; trypsinization
类别
资金
- Foundation of the National Natural Science [81673078]
- Clinical Medicine Science and Technology Projects of Jiangsu [BL 2014036]
Background: Autologous melanocyte transplantation plays an important role in the treatment of vitiligo. Objective: Previous studies have indicated that, compared with melanocytes growing in monolayers, melanocyte spheroids have a better survival in growth factor-and serum-deprived conditions. Methods: Melanocyte spheroids were obtained from human epidermis by repetitive long-term trypsinization and maintained an aggregated morphology for a short period in certain conditions. Results: Melanocyte spheroids were capable of growing into normal dendritic melanocytes in monolayer when they were harvested and reinoculated in 24-well plates. Immunohistochemical analysis of the melanocyte spheroids revealed that they were positive for HMB45, a melanosome-specific marker. No melanomas occurred when melanocyte spheroids were transplanted into mice. Conclusion: Our study provides a promising approach for melanocyte transplantation to treat vitiligo.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据