期刊
FRONTIERS IN MOLECULAR BIOSCIENCES
卷 6, 期 -, 页码 -出版社
FRONTIERS MEDIA SA
DOI: 10.3389/fmolb.2019.00028
关键词
DNA sequencing; xenonucleic acids; aptamers; functionalized DNA; unnatural base pairs
资金
- New England Biolabs
A variant of 9 degrees N DNA polymerase [Genbank ID (AAA88769.1)] with three mutations (D141A, E143A, A485L) and commercialized under the name Therminator DNA polymerase has the ability to incorporate a variety of modified nucleotide classes. This Review focuses on how Therminator DNA Polymerase has enabled new technologies in synthetic biology and DNA sequencing. In addition, we discuss mechanisms for increased modified nucleotide incorporation.
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