期刊
LIFE SCIENCES
卷 178, 期 -, 页码 9-16出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.lfs.2017.04.010
关键词
Ariboflavinosis; Adipocyte tissue; Inflammation; Obesity; Insulin resistance
资金
- Ministry of Science and Higher Education, Poland [IP2012 000872]
Aims: Adipose tissue is an endocrine organ important for regulation of such physiological processes as energy metabolism or lipids homeostasis. In an obesity state, it participates in the induction of chronic systemic inflammation accompanied by pro-inflammatory cytokines and fatty acid elevation. For this reasons, adipose tissue is involved in, e.g., insulin resistance, type 2 diabetes or hyperlipidemia development. In our previous study, we have shown that riboflavin deficiency induces a pathological pro-inflammatory response of macrophages, the main component of adipose tissue. Therefore, in the current study, we investigated the alteration of the pro inflammatory activity of adipocytes. Main methods: The study was conducted on mouse 3T3 L1 preadipocytes differentiated to adipocyte and culture in the state of riboflavin deficiency (3.1 nM) or control condition (10.4 nM). The cell viability, adiposity and glucose uptake was assessed. Moreover, mRNA expression, as well as crucial pro-inflammatory cytokines (TNF alpha, IL-6) and adipokines (adiponectin, leptin, resistin) release and NF kappa B activation, were evaluated. Key findings: Results showed that riboflavin deprivation induced a significant elevation in adipocyte lipolysis and enhance obesity-related apoptosis of adipocytes. The generation of reactive oxygen species was enhanced in riboflavin-deficient adipocytes by 43%. Moreover, NF kappa B phosphorylation and the expression and release of both TNF alpha, IL-6 as well as leptin were elevated in a deficient group what was accompanied by a reduction of adiponectin level. Conclusion: Our study shows that riboflavin deficiency can promote the intensification of pro-inflammatory activity of adipocyte cells, leading consequently to the severity of chronic inflammation that accompanies obesity state. (C) 2017 Elsevier Inc. All rights reserved.
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