期刊
LEUKEMIA RESEARCH
卷 55, 期 -, 页码 6-17出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.leukres.2017.01.008
关键词
IRF-1; Treg; Myelodysplastic syndrome; T regulatory cells; Foxp3; Immune system
资金
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) [2011/50910-8]
- Research and Development from Fleury Group [001/2012]
Studies have demonstrated that abnormalities in interferon regulatory factor-1 (IRF-1) expression might develop myelodysplastic syndromes (MDS). IRF-1 was described as modulator of T regulatory (Treg) cells by suppressing Foxp3 on mice. We aimed to determine the role of Treg and IRF-1 in MDS. Thirty-eight MDS patients fulfilling WHO criteria and classified according to risk scores were evaluated at time 0 (TO) and after 12 months (T12) for: Treg suppression activity in coculture with T effector (Teff) cells; IRF-1 and Foxp3 genetic expression by qRT-PCR; IL-2, 4, 6, 10, 17, TNF alpha. and IFN gamma production by Cytometric Bead Array. No differences in Foxp3 expression (T0 = 0.06 +/- 0.06 vs T12 = 0.06 +/- 0.12, p = 0.5), Treg number (TO = 5.62 +/- 2.84 x 10(5) vs T12 =4.87 +/- 2.62 x 10(5); p = 0.3) and Teff percentage (T0=16.8 +/- 9.56% vs T12 =13.1 +/- 6.3%; p = 0.06) were observed on T12. Low risk MDS patients showed a higher number of Treg (5.2 +/- 2.6 x 10(5)) versus high risk group (2.6 +/- 1.2 x 10(5), p = 0.03). Treg suppression activity was impaired on TO and T12.Cytokine production and IRF-1 expression were increased on T12. The correlation between IRF-1 and FoxP3 was negative (r(2) = 0.317, p = 0.045) on T0. These results suggest a hyper activity of the immune system, probably secondary to Treg suppression activity impairment. This state may induce the loss of tolerance culminating in the proliferation of MDS clones. (C) 2017 Elsevier Ltd. All rights reserved.
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