期刊
LETTERS IN APPLIED MICROBIOLOGY
卷 65, 期 4, 页码 281-284出版社
WILEY
DOI: 10.1111/lam.12778
关键词
biosecurity; decontamination; foot-and-mouth disease; hydrogen peroxide; virus
An evaluation was made of the efficacy of 35% hydrogen peroxide vapour (HPV) against foot-and-mouth disease virus (FMDV) in a biosafety facility. Biological indicators (BIs) were produced using three serotypes of FMDV, all with a titre of >= 10(6) TCID50 per ml. Fifteen BIs of each serotype were distributed across five locations, throughout a 30-m(3) airlock chamber, producing a total of 45 BIs. Thirty-five percent HPV was generated and applied using a Bioquell vaporization module located in the centre of the chamber. After a dwell period of 40min, the HPV was removed via the enclosures air handling system and the BIs were collected. The surfaces of the BIs were recovered into Glasgow's modified Eagle's medium (GMEM), cultivated in BHK21 Cl13 cell culture and analysed for evidence of cytopathic effect (CPE). No CPE was detected in any BI sample. Positive controls showed CPE. The experimentation shows that FMDV is susceptible to HPV decontamination and presents a potential alternative to formaldehyde.
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