期刊
ACS APPLIED BIO MATERIALS
卷 2, 期 5, 页码 2037-2049出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.9b00078
关键词
reconstructive surgery; decellularization; extracellular matrix (ECM); xenogenic cartilage matrix; caprine
资金
- Department of Biotechnology, DBT, Government of India [BT/PR7292/AAQ/534/2012]
- Department of Biotechnology (DBT)
- Department of Science and Technology (DST), Government of India
- West Bengal University of Animal and Fishery Sciences, Kolkata, India
Repair and regeneration of nasal and auricular cartilage thrust significant challenges in reconstructive surgery. The burgeoning clinical requirement is yet to endorse a satisfactory cartilage replacement matrix. In this regard, we have bioengineered cross-linked decellularized caprine conchal cartilage (DC) as biocompatible, durable, and nontoxic matrices. The DC matrices exhibited reduced DNA and sulfated glycosaminoglycan (sGAG) with a minimal effect on the collagen content. Further, histology and scanning electron micrographs revealed a significant loss of cellular bodies and the presence of a compact matrix consisting of intricate collagen fibers, when compared to unprocessed matrices. An in vitro biological assessment of the matrices exhibited an increased chondrocyte proliferation and viability with a significantly higher DNA, sGAG, and total collagen content. The matrices showed a 3-fold increase in the expression of cartilage-specific genes, namely, aggrecan, collagen II, and sox-9, and exhibited a minimal in vitro immunogenicity. Further, an in vivo assessment was performed by xenografting these caprine matrices in a rabbit model. The retrieved matrices showed a well-organized structural and cellular orientation with extracellular matrix formation after 3 months of implantation. No significant infiltration of plasma cells, macrophages, lymphocytes, and immature fibroblasts was recorded. Therefore, these affordable, resourceful, xenocompatible matrices offer a potential alternate in the repair and regeneration of nasal and auricular cartilages.
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