Proton density-weighted laryngeal magnetic resonance imaging in systemically dehydrated rats

Objectives/HypothesisDehydrated vocal folds are inefficient sound generators. Although systemic dehydration of the body is believed to induce vocal fold dehydration, this causative relationship has not been demonstrated in vivo. Here we investigate the feasibility of using in vivo proton density (PD)-weighted magnetic resonance imaging (MRI) to demonstrate hydration changes in vocal fold tissue following systemic dehydration in rats. Study DesignAnimal study. MethodsSprague-Dawley rats (n=10) were imaged at baseline and following a 10% reduction in body weight secondary to withholding water. In vivo, high-field (7T), PD-weighted MRI was used to successfully resolve vocal fold and salivary gland tissue structures. ResultsNormalized signal intensities within the vocal fold decreased postdehydration by an average of 11.38%3.95% (meanstandard error of the mean [SEM], P=.0098) as compared to predehydration levels. The salivary glands experienced a similar decrease in normalized signal intensity by an average of 10.74%+/- 4.14% (mean +/- SEM, P=.0195) following dehydration. The correlation coefficient (percent change from dehydration) between vocal folds and salivary glands was 0.7145 (P=.0202). ConclusionsTen percent systemic dehydration induced vocal fold dehydration as assessed by PD-weighted MRI. Changes in the hydration state of vocal fold tissue were highly correlated with that of the salivary glands in dehydrated rats in vivo. These preliminary findings demonstrate the feasibility of using PD-weighted MRI to quantify hydration states of the vocal folds and lay the foundation for further studies that explore more routine and realistic magnitudes of systemic dehydration and rehydration. Level of EvidenceNA. Laryngoscope, 128:E222-E227, 2018