期刊
INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES
卷 15, 期 4, 页码 857-869出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijbs.30611
关键词
miRNA-322; Rassf8; SSCs; slef-renewal; WNT/beta-catenin signaling pathway
资金
- National Basic Research Program of China [2018YFC1003501]
- National Nature Science Foundation of China [81720108017]
Spermatogonial stem cells (SSCs) are essential for spermatogenesis and male fertility. MicroRNAs (miRs) are key regulators of gene expression involved in self-renewal, differentiation, and apoptosis. However, the function and mechanisms of individual miR in regulating self-renewal and differentiation of SSCs remain unclear. Here, we report for the first time that miR-322 regulates self-renewal of SSCs. Functional assays revealed that miR-322 was essential for SSC self-renewal. Mechanistically, miR-322 promoted SSC self-renewal by targeting RASSF8 (ras association domain family 8). Moreover, the WNT/beta-catenin signaling pathway was involved in the miR-322-mediated regulation. Furthermore, miR-322 overexpression increased GFR alpha l, ETV5 and PLZF expression but decreased STRA8, C-KIT and BCL6 expression. Our study provides not only a novel insight into molecular mechanisms regulating SSC self-renewal but also a basis for the diagnosis, treatment, and prevention of male infertility.
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