期刊
LAB ON A CHIP
卷 17, 期 9, 页码 1594-1602出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c7lc00170c
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资金
- SCIEX, Abbott Laboratories
- Natural Sciences and Engineering Research Council (NSERC)
- Canadian Institutes for Health Research (CIHR)
- Canadian Breast Cancer Foundation (CBCF)
- Ministry of Higher Education of Egypt
Despite advances in breast cancer prevention and treatment, variability in patient-response has revealed the need for a more personalized approach to medicine, in which treatments are tailored to each patient's biology. Motivated by this idea, we introduce a technique that allows for quantification of small-molecule analytes directly from core needle biopsy (CNB) tissue samples on a miniaturized platform. The new technique, powered by digital microfluidics, integrates tissue-liquid extraction and magnetic bead-based competitive immunoassay for quantification of estradiol in milligram-sized CNB samples. Each measurement (from start to finish) requires similar to 40 minutes, a duration consistent with a visit to a doctor's office. The performance of the new technique was validated by the gold-standard analysis method (high performance liquid chromatography coupled to tandem mass spectrometry), and was applied to evaluate human patient samples before and after a course of treatment with aromatase inhibitor therapy. We propose that the new technique has great potential for eventual use for fast, automated, and quantitative analysis of bio-markers in tissue samples, towards a personalized medicine approach.
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